· Definitions
· Hand hygiene
· Attire
· Equipment classification
· Anaesthetic equipment
· Sharps
Asepsis |
· Prevention of microbial contamination of sterile tissues and objects |
Decontamination |
· Killing microbes to render an object safe for use |
-Cleaning |
· Remove organic matter and kill many microbes |
-Disinfection |
· Kill all microbes except spores |
-Sterilisation |
· Kill all microbes |
If soiled |
· Wash hands with soap |
If not soiled |
· Anti-microbial solution: o Alcohol 60-95% (kills microbes) o Chlorhex 2-4% (preventing regrowth) o Water (required for denaturing) |
N.B. the most important infection control measure
In theatre |
· Scrubs · Shoes · Hat |
Body fluid contact |
· Gown · Mask · Goggles · Gloves |
Critical |
· Penetrates skin or mucous membranes
-> cleaning then sterilization |
Semi-critical |
· Touches mucous membranes
-> cleaning then disinfection |
Non-critical |
· Touches skin
-> cleaning only |
Airway |
· Face mask: semi-critical · Laryngoscope blade: critical · Laryngoscope handle: non-critical (but disinfect if bloody) · Flexible bronchoscopes: semi-critical |
Vascular access |
PIVC: · Alcohol if <24 hours, alcohol + chlorhex if >24 hours · No evidence that cannulas should be routinely replaced CVC: · Proceduralist: hat, facemask, sterile gown and gloves · Patient: alcohol + chlorhex + sterile full body drape Other: · Ports: alcohol + allow to dry |
Regional |
· Ensure chlorhex cannot be injected into neural tissue · Neuraxial and regional catheter: as for CVC · Regional single shot: sterile gloves + no-touch technique · If ultrasound: sterile gel + sterile needle entry site |
Drugs |
· Beware preservative-containing solutions · Beware the outside of an ampoule: it is dirty · Beware the rubber seal of a vial: it is not sterile · Only 20μm filter needles effectively exclude microbes · Ampoule splitting increases risk of infection and error |
Machine |
· Machine: periodic decontamination · Tubing: new filter per patient, new tubing if respiratory virus · Breathing bag: non-critical · Sampling line: re-usable if connected to the circuit via a filter |
Ultrasound |
· Diagnostic: non-critical, bare probe ok, needs cleaning · Interventional: semi-critical, needs probe cover, needs disinfection · Internal: semi-critical (N.B. probe, shaft, socket, handle, cable) · Bare probe + body fluid = needs sterilization |
Disposal |
· Into sharps bin · Immediately after use · No re-sheathing or bending |
Needle-stick injuries |
· Risk of HBV 30%, HCV 3%, HIV 0.3% if virus present · Wash with water and antiseptic · Report ASAP to infection control team · All staff should be vaccinated against HBV |
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